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Detailed information for vg0729627357:

Variant ID: vg0729627357 (JBrowse)Variation Type: INDEL
Chromosome: chr07Position: 29627357
Reference Allele: AGAACGTTGAlternative Allele: A
Primary Allele: AGAACGTTGSecondary Allele: A

Inferred Ancestral Allele : AGAACGTTG (evidence from allele frequency in Oryza rufipogon: AGAACGTTG: 1.00, others allele: 0.00, population size: 132. )

Flanking Sequence (100 bp) in Reference Genome:


ACGGATTCTGCTTACAACATGAAGTCAAACTCAGATGCTGCACCAATAAAACAAGGTTCTAATGGTAGTAGCAATAACAATGACATGGGTTCCACTACAA[AGAACGTTG/A]
TGACAAAGCCTAGTACAAATAAGGAGAGAGTAATGTCACCCTCAGCTGTTAAGGCTAATGGACACACATCAGCATTTCATCCTGCACAGCACTGGACGTC

Reverse complement sequence

GACGTCCAGTGCTGTGCAGGATGAAATGCTGATGTGTGTCCATTAGCCTTAACAGCTGAGGGTGACATTACTCTCTCCTTATTTGTACTAGGCTTTGTCA[CAACGTTCT/T]
TTGTAGTGGAACCCATGTCATTGTTATTGCTACTACCATTAGAACCTTGTTTTATTGGTGCAGCATCTGAGTTTGACTTCATGTTGTAAGCAGAATCCGT

Allele Frequencies:

Populations Population SizeFrequency of AGAACGTTG(primary allele) Frequency of A(secondary allele) Frequency of N Frequency of DEL Frequency of others Allele
All  4726 90.90% 6.70% 2.35% 0.00% NA
All Indica  2759 84.80% 11.30% 3.84% 0.00% NA
All Japonica  1512 99.60% 0.20% 0.20% 0.00% NA
Aus  269 99.30% 0.00% 0.74% 0.00% NA
Indica I  595 50.40% 38.20% 11.43% 0.00% NA
Indica II  465 91.00% 4.90% 4.09% 0.00% NA
Indica III  913 100.00% 0.00% 0.00% 0.00% NA
Indica Intermediate  786 89.60% 8.00% 2.42% 0.00% NA
Temperate Japonica  767 99.60% 0.40% 0.00% 0.00% NA
Tropical Japonica  504 100.00% 0.00% 0.00% 0.00% NA
Japonica Intermediate  241 98.80% 0.00% 1.24% 0.00% NA
VI/Aromatic  96 100.00% 0.00% 0.00% 0.00% NA
Intermediate  90 98.90% 1.10% 0.00% 0.00% NA

QTN in RiceNavi

Rice quantitative trait nucleotides (QTNs) and inferred QTN effects are from Wei et al., Nature Genetics, 2021.

CategoryVariant IDChromPosGeneMSURAPAlt_Allele_FunctionRef_genoAlt_geno
Heading datevg0729627357Chr729627357OsPRR37/Hd2/Ghd7.1LOC_Os07g49460Os07g0695100promoting heading date under LDAGAACGTTGA

Allele Effect:

Var ID Var Locus snpEff Annotation CooVar Annotation Chromatin Accessibility Score PolyPhen-2 Effect PolyPhen-2 Score SIFT Effect SIFT Score
vg0729627357 AGAACGTTG -> A LOC_Os07g49460.1 frameshift_variant ; p.Lys505fs; HIGH frameshift_variant Average:75.016; most accessible tissue: Minghui63 flag leaf, score: 88.687 N N N N
vg0729627357 AGAACGTTG -> A LOC_Os07g49460.3 frameshift_variant ; p.Lys505fs; HIGH frameshift_variant Average:75.016; most accessible tissue: Minghui63 flag leaf, score: 88.687 N N N N
vg0729627357 AGAACGTTG -> A LOC_Os07g49460.4 frameshift_variant ; p.Lys505fs; HIGH frameshift_variant Average:75.016; most accessible tissue: Minghui63 flag leaf, score: 88.687 N N N N
vg0729627357 AGAACGTTG -> A LOC_Os07g49460.2 frameshift_variant ; p.Lys505fs; HIGH frameshift_variant Average:75.016; most accessible tissue: Minghui63 flag leaf, score: 88.687 N N N N

Effects Predicted by Deep Convolutional Neural Networks

For each variant, we constructed two sequences that contain the variation site and the sequence around it, differing only in the variation site. We then used Basenji to predict the chromatin accessibility of each tissue for the two sequences, respectively, and scored the effect of the variant by comparing the changes in chromatin accessibility corresponding to the two genotypes in the 1 kb region around the variation site. The effect score was defined as the logarithmic ratio of the predicted chromatin accessibility of the alternative genotype to the value of the reference genotype.

Var ID Ref Alt Root (RT) Young Leaf (YL) Flag Leaf (FL) Young Panicle (YP) Lemma & Palea (LP) Stamen & Pistil (SP)
vg0729627357 AGAAC* A 0.05 -0.13 -0.02 0.08 0.12 0.03